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pLV hUbC-VP64 dCas9 VP64-T2A-GFP
(Plasmid #59791)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59791 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FUGW
  • Backbone size w/o insert (bp) 10000
  • Total vector size (bp) 15000
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    humanized VP64 dead Cas9 VP64 T2A GFP
  • Species
    S. Pyogenes
  • Mutation
    D10A and H840A
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV hUbC-VP64 dCas9 VP64-T2A-GFP was a gift from Charles Gersbach (Addgene plasmid # 59791)
  • For your References section:

    Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA. Nucleic Acids Res. 2014 Aug 13. pii: gku749. 10.1093/nar/gku749 PubMed 25122746