pGL2 HNF4A P2 mut
PurposeMutated HNF4A P2 promoter region cloned into the pGL2-basic backbone. The -181 G to A mutation is in a HNF1alpha binding site and cosegregates with MODY.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60325||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5598
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameHNF4A P2 mut (-181 G to A)
SpeciesH. sapiens (human)
MutationThe -181 G to A mutation is in a HNF1alpha binding site and cosegregates with MODY.
Entrez GeneHNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14)
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (not destroyed)
- 3′ cloning site Unknown (not destroyed)
- 5′ sequencing primer GLprimer2 (Promega): CTTTATGTTTTTGGCGTCTTCCA (Common Sequencing Primers)
–371 to –37 from HNF4A TSS
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2 HNF4A P2 mut was a gift from Jorge Ferrer (Addgene plasmid # 60325 ; http://n2t.net/addgene:60325 ; RRID:Addgene_60325)
For your References section:Genetic evidence that HNF-1alpha-dependent transcriptional control of HNF-4alpha is essential for human pancreatic beta cell function. Hansen SK, Parrizas M, Jensen ML, Pruhova S, Ek J, Boj SF, Johansen A, Maestro MA, Rivera F, Eiberg H, Andel M, Lebl J, Pedersen O, Ferrer J, Hansen T. J Clin Invest. 2002 Sep;110(6):827-33. 10.1172/JCI15085 PubMed 12235114