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pEGFP-N1-FLAG
(Plasmid #60360)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60360 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 60360-DNA.cg 2 µg of cloning grade DNA in Tris buffer
More Information
1 $95

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 4733
  • Modifications to backbone
    Insertion of a FLAG tag in front of GFP together with an ATG and a strong kozak sequence.
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • EGFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Cloning Information

Resource Information

Information for Cloning Grade DNA (Catalog # 60360-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Resource Information

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-FLAG was a gift from Patrick Calsou (Addgene plasmid # 60360 ; http://n2t.net/addgene:60360 ; RRID:Addgene_60360)
  • For your References section:

    DNA damage triggers SAF-A and RNA biogenesis factors exclusion from chromatin coupled to R-loops removal. Britton S, Dernoncourt E, Delteil C, Froment C, Schiltz O, Salles B, Frit P, Calsou P. Nucleic Acids Res. 2014 Oct 1;42(14):9047-62. doi: 10.1093/nar/gku601. Epub 2014 Jul 16. 10.1093/nar/gku601 PubMed 25030905