Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pGEX-6P-1 SARS-CoV 3CLpro
(Plasmid #61692)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61692 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4984
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS 3CLpro protease
  • Species
    SARS
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • HIS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

For protein expression, grow in Rosetta cells.
25 degree induction for 14-15 hours with 0.5 mM IPTG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P-1 SARS-CoV 3CLpro was a gift from Michael Johnson (Addgene plasmid # 61692 ; http://n2t.net/addgene:61692 ; RRID:Addgene_61692)
  • For your References section:

    Identification of novel drug scaffolds for inhibition of SARS-CoV 3-Chymotrypsin-like protease using virtual and high-throughput screenings. Lee H, Mittal A, Patel K, Gatuz JL, Truong L, Torres J, Mulhearn DC, Johnson ME. Bioorg Med Chem. 2014 Jan 1;22(1):167-77. doi: 10.1016/j.bmc.2013.11.041. Epub 2013 Dec 1. 10.1016/j.bmc.2013.11.041 PubMed 24332657