pET15b SARS-CoV PLpro
Purposeinducible expression of SARS PLpro protease
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61693||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 5708
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameSARS-CoV PLpro
- Promoter T7
/ Fusion Protein
- His (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
For protein expression, grow in Rosetta cells.
25 degree induction for 14-15 hours with 0.5 mM IPTG
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET15b SARS-CoV PLpro was a gift from Michael Johnson (Addgene plasmid # 61693 ; http://n2t.net/addgene:61693 ; RRID:Addgene_61693)
For your References section:Synergistic inhibitor binding to the papain-like protease of human SARS coronavirus: mechanistic and inhibitor design implications. Lee H, Cao S, Hevener KE, Truong L, Gatuz JL, Patel K, Ghosh AK, Johnson ME. ChemMedChem. 2013 Aug;8(8):1361-72. doi: 10.1002/cmdc.201300134. Epub 2013 Jun 20. 10.1002/cmdc.201300134 PubMed 23788528