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pAAV-UbC-tdTomato
(Plasmid #62516)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62516 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-SIBR-anti_I
  • Backbone manufacturer
    Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
  • Backbone size w/o insert (bp) 5212
  • Total vector size (bp) 6661
  • Modifications to backbone
    The anti-luciferase SIBR and eGFP sequence was deleted from the original backbone and new polylinker was created in the place of eGFP.
  • Vector type
    Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Species
    Was optimized on Human codon usage
  • Insert Size (bp)
    1449
  • Promoter hUbC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCAG_F_primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The tdTomato was constructed synthetically based on the Human codon usage.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-UbC-tdTomato was a gift from Jae Lee & Pantelis Tsoulfas (Addgene plasmid # 62516 ; http://n2t.net/addgene:62516 ; RRID:Addgene_62516)
  • For your References section:

    3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates. Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, Lemmon VP, Lee JK, Tsoulfas P. Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015. 10.1523/ENEURO.0001-15.2015 PubMed 26023683