PurposeExpresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62516||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerChung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
- Backbone size w/o insert (bp) 5212
- Total vector size (bp) 6661
Modifications to backboneThe anti-luciferase SIBR and eGFP sequence was deleted from the original backbone and new polylinker was created in the place of eGFP.
Vector typeMammalian Expression, AAV, Synthetic Biology
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesWas optimized on Human codon usage
Insert Size (bp)1449
- Promoter hUbC
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pCAG_F_primer (Common Sequencing Primers)
The tdTomato was constructed synthetically based on the Human codon usage.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-UbC-tdTomato was a gift from Jae Lee & Pantelis Tsoulfas (Addgene plasmid # 62516 ; http://n2t.net/addgene:62516 ; RRID:Addgene_62516)
For your References section:3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates. Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, Lemmon VP, Lee JK, Tsoulfas P. Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015. 10.1523/ENEURO.0001-15.2015 PubMed 26023683