pAAV-UbC-eGFP
(Plasmid #62518)

• Purpose: Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.
• Depositing Labs: Jae Lee, Pantelis Tsoulfas
• Publication: Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015.

Backbone

• Vector backbone: pAAV-SIBR-anti_l
• Backbone manufacturer: Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
• Backbone size w/o insert (bp): 5223
• Total vector size (bp): 5937
• Modifications to backbone: The anti-luciferase cassette was deleted
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eGFP
• Alt name: enhanced green fluorescent protein
• Species: Aqueora Victoria
• Insert Size (bp): 714
• Promoter: hUbC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: pCAG_F_primer
• 3′ sequencing primer: EGFP_C_primer

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry