Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAAV-UbC-eGFP
(Plasmid #62518)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62518 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-SIBR-anti_l
  • Backbone manufacturer
    Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
  • Backbone size w/o insert (bp) 5223
  • Total vector size (bp) 5937
  • Modifications to backbone
    The anti-luciferase cassette was deleted
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Alt name
    enhanced green fluorescent protein
  • Species
    Aqueora Victoria
  • Insert Size (bp)
    714
  • Promoter hUbC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer pCAG_F_primer
  • 3′ sequencing primer EGFP_C_primer
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-UbC-eGFP was a gift from Jae Lee & Pantelis Tsoulfas (Addgene plasmid # 62518 ; http://n2t.net/addgene:62518 ; RRID:Addgene_62518)
  • For your References section:

    3D Imaging of Axons in Transparent Spinal Cords from Rodents and Nonhuman Primates. Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, Lemmon VP, Lee JK, Tsoulfas P. Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015. 10.1523/ENEURO.0001-15.2015 PubMed 26023683