Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAAV-UbC-eGFP-F
(Plasmid #71545)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71545 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-SIBR-anti_I
  • Backbone manufacturer
    Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
  • Backbone size w/o insert (bp) 5235
  • Total vector size (bp) 6048
  • Modifications to backbone
    The anti-luciferase cassette was deleted and eGFP was replaced with eGFP that has in the COOH terminus a 20- amino-acid farnesylation signal from c-Ha-Ras. The gene was synthesized for optimal mammalian cell expression
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP-F
  • Alt name
    membrane bound eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    813
  • GenBank ID
    AEO31555.1 JN255744.1
  • Promoter UbC
  • Tag / Fusion Protein
    • contains 20- amino-acid farnesylation signal from c-Ha-Ras and a 4 aminoacid linker (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer common sequencing primers
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-UbC-eGFP-F was a gift from Pantelis Tsoulfas (Addgene plasmid # 71545 ; http://n2t.net/addgene:71545 ; RRID:Addgene_71545)