pCMV CytoBAS
(Plasmid #62860)

• Purpose: Fluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET
• Depositing Lab: Stan van de Graaf
• Publication: van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi: 10.1002/hep.26012. Epub 2013 Jan 8.

Backbone

• Vector backbone: pECFP-C1
• Backbone manufacturer: Clontech
• Total vector size (bp): 6251
• Modifications to backbone: Synthetic DNA construct encoding BAS-1 was cloned into pECFP-C1, replacing ECFP, to generate NucleoBAS. CytoBAS was created by KpnI digestion of NucleoBAS, which removed the NLS.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CytoBAS
• Species: Synthetic
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry