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pKG204
(Plasmid #63078)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63078 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIC242
  • Backbone manufacturer
    Cheeseman/Desai (Addgene plasmid #44432)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HP1 alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    576
  • Mutation
    V151I mutation compared to GenBank reference sequence NP_036249.1
  • Entrez Gene
    CBX5 (a.k.a. HEL25, HP1, HP1A)
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • TEV-S (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer oCB21
  • 3′ sequencing primer oCB22
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG204 was a gift from Iain Cheeseman (Addgene plasmid # 63078 ; http://n2t.net/addgene:63078 ; RRID:Addgene_63078)