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Iain Cheeseman Lab: Cheeseman Lab Plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
63061pIC65Retroviral expression of EGFP-TEV-S-tag Mis12 for protein localization and affinity purification (LAP)
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63062pIC84Retroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)
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63063pIC79Mammalian expression of EGFP-TEV-S-tag hNSL1 for protein localization and affinity purification (LAP)
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63064pIC80Mammalian expression of EGFP-TEV-S-tag DSN1 for protein localization and affinity purification (LAP)
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63065pIC81Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)
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63067pIC144Retroviral expression of EGFP-TEV-S-tag CENP-K for protein localization and affinity purification (LAP)
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63068pIC145Retroviral expression of EGFP-TEV-S-tag CENP-O for protein localization and affinity purification (LAP)
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63069pIC146Retroviral expression of EGFP-TEV-S-tag CENP-P for protein localization and affinity purification (LAP)
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63070pIC162Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein localization and affinity purification (LAP)
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63072pKG96Retroviral expression of EGFP-TEV-S-tag CENP-A for protein localization and affinity purification (LAP)
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63073pKG113Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
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63074pKG123Retroviral expression of mCherry-TEV-S-tag CENP-C for protein localization and affinity purification (LAP)
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63075pKG136Retroviral expression of EGFP-TEV-S-tag CENP-W for protein localization and affinity purification (LAP)
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63076pKG186Retroviral expression of EGFP-TEV-S-tag FANC-A for protein localization and affinity purification (LAP)
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63077pKG187Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)
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63078pKG204Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)
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69730pIC195Retroviral expression of Polo-Like Kinase 1 (Plk1)
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69732pIC301Retroviral expression of Bub1
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69733pIC234Retroviral expression of Nup-37
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69735pIC214Mammalian Expression of Tousled-like kinase 1 (TLK1)
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69736pIC215Mammalian Expression of PELP1
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69737pIC232Retroviral expression of Bod1
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69738pIC235Retroviral expression of CENP-R
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69739pIC241Retroviral expression of Sgo-1
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69741pIC247 Retroviral expression of Borealin
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69743pCB94Retroviral expression of PP2A beta
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69744pCB98Retroviral expression of Centrin 1
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69745pCB99Retroviral expression of Centrin 2
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69746pCB100Retroviral expression of Centrin 3
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69747pCB52Retroviral expression of Cyclin B1
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69748pCB265Retroviral expression of HURP
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69751pKG333Retroviral expression of Histone H3
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69752pKG422Retroviral expression of spc24
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69753pKG326Retroviral expression of CENP-T aa 1-531
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69757pKG194Mammalian Expression of CENP-T aa 1-375
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69758pKG212Retroviral expression of CENP-T aa 1-242
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69759pKG141Retroviral expression of CENP-B
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69760pKG402Retroviral expression of CENP-T aa 256-561
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69761pKG210Retroviral expression of CENP-T aa 1-375
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69762pKM343Retroviral expression of CENP-I
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134181pKM808Lentiviral sgRNA plasmid with blasticidin resistance
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134190pDK324Expression of C. elegans KNL1 with 8A mutation
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134194pDK362Retroviral plasmid with SKAP insert
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134195pDK365Retroviral plasmid with SKAP insert
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134202pDK616Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant
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134203pDK637bacterial expression of human CENP-N 1-240
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134205pDK580Baculovirus expresion of CENP-L and CENP-N
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134207pDK712Mammalian expression of EB3-tDT BMAM
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134226pKM28Retroviral plasmid expressing mCherry - HJURP
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134227pKM510Retroviral plasmid expressing mCherry - HJURP
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134228pFR022bacterial expression of KNL1
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134229pDK713Mammalian expresion of tdTomato SKAP
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134230pDK714Mammalian expresion of tdTomato SKAP
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134246HP138-puroCas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.
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134247HP138-neoCas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection
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