pGEX4T-E2-25K
(Plasmid
#63572)
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PurposeExpression of human Ubiquitin conjugating enzyme E2-25K
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX4T
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUBE2-25K
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SpeciesH. sapiens (human)
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Insert Size (bp)645
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MutationIsoform 1 (200 resiudes)
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Entrez GeneUBA7 (a.k.a. D8, UBA1B, UBE1L, UBE2)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX5
- 3′ sequencing primer pGEX3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX4T-E2-25K was a gift from Titia Sixma (Addgene plasmid # 63572 ; http://n2t.net/addgene:63572 ; RRID:Addgene_63572) -
For your References section:
SUMO modification of the ubiquitin-conjugating enzyme E2-25K. Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK. Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. 10.1038/nsmb903 PubMed 15723079