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MSGV Hu Acceptor PGK-NGFR
(Plasmid #64270)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64270 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMSGV
  • Backbone manufacturer
    Richard Morgan NCI
  • Backbone size (bp) 8070
  • Modifications to backbone
    TCR cloning insert: TCR V region gene acceptor/constant region genes tNGFR driven by PGK promoter
  • Vector type
    Retroviral
  • Selectable markers
    tNGFR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSGV Hu Acceptor PGK-NGFR was a gift from David Ott (Addgene plasmid # 64270 ; http://n2t.net/addgene:64270 ; RRID:Addgene_64270)
  • For your References section:

    Production of retroviral constructs for effective transfer and expression of T-cell receptor genes using Golden Gate cloning. Coren LV, Jain S, Trivett MT, Ohlen C, Ott DE. Biotechniques. 2015 Mar 1;58(3):135-9. doi: 10.2144/000114265. eCollection 2015. 10.2144/000114265 PubMed 25757546