Purpose(Empty Backbone) lentiviral vector with Flag, HA tags
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64874||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||64874-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Backbone manufacturerSystem Biosciences
Modifications to backboneAddition of FLAG and HA tags
Vector typeMammalian Expression, Lentiviral
/ Fusion Proteins
- Flag (C terminal on backbone)
- HA (C terminal on backbone)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF1a-F_alt (gccgtgaacgttctttttc)
- 3′ sequencing primer IRES-R (CCTCACATTGCCAAAAGACG) (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 64874-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDH-EF1-FHC was a gift from Richard Wood (Addgene plasmid # 64874 ; http://n2t.net/addgene:64874 ; RRID:Addgene_64874)
For your References section:Mechanism of suppression of chromosomal instability by DNA polymerase POLQ. Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, Bylund GO, Doublie S, Johansson E, Ramsden DA, McBride KM, Wood RD. PLoS Genet. 2014 Oct 2;10(10):e1004654. doi: 10.1371/journal.pgen.1004654. eCollection 2014 Oct. PGENETICS-D-14-01461 [pii] PubMed 25275444