Purposephotoactivatable PKA inhibition
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65456||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 5227
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer CMV-F or GFP-F (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mVenus-PA-PKI was a gift from Klaus Hahn (Addgene plasmid # 65456 ; http://n2t.net/addgene:65456 ; RRID:Addgene_65456)
For your References section:Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. Yi JJ, Wang H, Vilela M, Danuser G, Hahn KM. ACS Synth Biol. 2014 Nov 21;3(11):788-95. doi: 10.1021/sb5001356. Epub 2014 Jun 13. 10.1021/sb5001356 PubMed 24905630