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pAc.1B-EGFP-DmHrb98
(Plasmid #66595)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66595 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6064
  • Total vector size (bp) 7147
  • Modifications to backbone
    N-terminal EGFP-tag
  • Vector type
    Drosophila Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hrb98
  • Alt name
    CG9983
  • Alt name
    hnRNPA2/B1
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1083
  • GenBank ID
    NM_170371
  • Entrez Gene
    Hrb98DE (a.k.a. Dmel_CG9983, CG9983, Dmel\CG9983, E7Delta6, HDP(P9), HRB98DE, HRB98DE/hrp38, Hrb1, Hrb98 de, Hrb98DE/Hrp38, Hrp38, Pen9, anon-E7Delta6, hnRNP A2/B1, hrb98 de, hrb98DE, hrp38, p9)
  • Promoter Actin 5C
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EGFP-forw
  • 3′ sequencing primer Bgh-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc.1B-EGFP-DmHrb98 was a gift from Elisa Izaurralde (Addgene plasmid # 66595 ; http://n2t.net/addgene:66595 ; RRID:Addgene_66595)