PurposemKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66826||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepUC19 (modified)
- Backbone size w/o insert (bp) 2600
- Total vector size (bp) 10500
Modifications to backboneAddition of ccdB markers to facilitate homology arm cloning
Vector typeWorm Expression, Cre/Lox, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Turbo
Copy numberHigh Copy
SpeciesC. elegans (nematode), Synthetic
Insert Size (bp)6600
/ Fusion Proteins
- C. elegans codon-optimized mKate2
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Forward (tgtaaaacgacggccagt) (Common Sequencing Primers)
THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD287 (https://www.addgene.org/70685/). The full sequence for this plasmid can still be found by clicking the link next to "Depositor Sequences".
IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDD285 was a gift from Bob Goldstein (Addgene plasmid # 66826)
For your References section:Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B. Genetics. 2015 Jun 3. pii: genetics.115.178335. 10.1534/genetics.115.178335 PubMed 26044593
Map uploaded by the depositor.