PurposeCRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61250||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerGoldstein lab
- Backbone size w/o insert (bp) 8113
- Total vector size (bp) 8148
Modifications to backboneOriginal sgRNA has been replaced with the improved synthetic guide RNA, sgRNA(F+E) (Chen et al., 2013, Cell). Contains an sgRNA sequence targeting the Y61A9LA.1 gene (Friedland et al., 2013, Nature Methods).
Vector typeWorm Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)123
- Promoter U6 promoter
- Cloning method Gibson Cloning
- 5′ sequencing primer attgtgttcgttgagtgaccc
- 3′ sequencing primer ggtgtgaaataccgcacaga (Common Sequencing Primers)
target sequence: ggatggatgtgtagtcaatt
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJW1219 was a gift from Jordan Ward (Addgene plasmid # 61250 ; http://n2t.net/addgene:61250 ; RRID:Addgene_61250)
For your References section:Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair. Ward JD. Genetics. 2014 Dec 9. pii: genetics.114.172361. 10.1534/genetics.114.172361 PubMed 25491644