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pJW1285
(Plasmid #61252)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61252 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJW1219
  • Backbone manufacturer
    Jordan Ward
  • Backbone size w/o insert (bp) 8146
  • Total vector size (bp) 8146
  • Modifications to backbone
    Replaced the sgRNA target sequence in the backbone with a sequence targeting the pha-1 locus near the e2123 allele.
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA(F+E) targeting pha-1
  • Species
    Synthetic
  • Insert Size (bp)
    123

Resource Information

Depositor Comments

Target sequence: atgaataacttgatgaacat

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW1285 was a gift from Jordan Ward (Addgene plasmid # 61252)
  • For your References section:

    Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair. Ward JD. Genetics. 2014 Dec 9. pii: genetics.114.172361. 10.1534/genetics.114.172361 PubMed 25491644