Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
Ward JD
Genetics. 2014 Dec 9. pii: genetics.114.172361. PubMed Article
Ward JD
Genetics. 2014 Dec 9. pii: genetics.114.172361. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61250 | pJW1219 | CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans |
| 61251 | pJW1259 | Cas9 plasmid with sgRNA deleted; for use in C. elegans |
| 61252 | pJW1285 | CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans |
| 61253 | pJW1310 | Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion |
| 61254 | pJW1311 | Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion |