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pa-G-GECO1.2
(Plasmid #67622)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67622 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV
  • Backbone manufacturer
    Campbell RE
  • Backbone size w/o insert (bp) 3200
  • Total vector size (bp) 4457
  • Modifications to backbone
    Customized Vector, see Addgene # 32446, Zhao et al Science. 2011.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pa-G-GECO1.2
  • Alt name
    photoactivatable G-GECO1.2
  • Species
    Synthetic
  • Insert Size (bp)
    1257
  • Mutation
    V81H, M84K, L189F, T190S

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    clone purchased from Addgene, engineered by Robert Campbell. Publication: Zhao et al Science. 2011 Sep 8.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pa-G-GECO1.2 was a gift from Ehud Isacoff (Addgene plasmid # 67622 ; http://n2t.net/addgene:67622 ; RRID:Addgene_67622)
  • For your References section:

    Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging. Berlin S, Carroll EC, Newman ZL, Okada HO, Quinn CM, Kallman B, Rockwell NC, Martin SS, Lagarias JC, Isacoff EY. Nat Methods. 2015 Jul 13. doi: 10.1038/nmeth.3480. 10.1038/nmeth.3480 PubMed 26167640