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pHMM
(Plasmid #67717)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 67717 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET30b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5422
  • Total vector size (bp) 6500
  • Modifications to backbone
    Insertion of sequence between NdeI and HindIII sites
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hexahistidine/Maltose binding protein/MS2 coat protein fusion
  • Species
    Synthetic
  • Insert Size (bp)
    1000
  • Promoter T7 RNA polymerase
  • Tag / Fusion Protein
    • Hexahistidine tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The MS2 coat protein in this plasmid contains the V29/dIFG mutations described in Lim and Peabody 1994 (Nucleic Acids Res. 1994 Sep 11;22(18):3748-52) which are reported to prevent coat protein multimerization and increase affinity for RNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHMM was a gift from Robert Batey & Jeffrey Kieft (Addgene plasmid # 67717 ; http://n2t.net/addgene:67717 ; RRID:Addgene_67717)
  • For your References section:

    Improved native affinity purification of RNA. Batey RT, Kieft JS. RNA. 2007 Aug;13(8):1384-9. Epub 2007 Jun 4. 10.1261/rna.528007 PubMed 17548432