Cas9 sgRNA vector Citations (16)
Originally described in: Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9.Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. PubMed Journal
Articles Citing Cas9 sgRNA vector
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| PSEN1 Mutant iPSC-Derived Model Reveals Severe Astrocyte Pathology in Alzheimer's Disease. Oksanen M, Petersen AJ, Naumenko N, Puttonen K, Lehtonen S, Gubert Olive M, Shakirzyanova A, Leskela S, Sarajarvi T, Viitanen M, Rinne JO, Hiltunen M, Haapasalo A, Giniatullin R, Tavi P, Zhang SC, Kanninen KM, Hamalainen RH, Koistinaho J. Stem Cell Reports. 2017 Dec 12;9(6):1885-1897. doi: 10.1016/j.stemcr.2017.10.016. Epub 2017 Nov 16. PubMed |
| Generation of a rod-specific NRL reporter line in human pluripotent stem cells. Phillips MJ, Capowski EE, Petersen A, Jansen AD, Barlow K, Edwards KL, Gamm DM. Sci Rep. 2018 Feb 5;8(1):2370. doi: 10.1038/s41598-018-20813-3. PubMed |
| Glucosylceramidase Maintains Influenza Virus Infection by Regulating Endocytosis. Drews K, Calgi MP, Harrison WC, Drews CM, Costa-Pinheiro P, Shaw JJP, Jobe KA, Nelson EA, Han JD, Fox T, White JM, Kester M. J Virol. 2019 May 29;93(12). pii: JVI.00017-19. doi: 10.1128/JVI.00017-19. Print 2019 Jun 15. PubMed |
Programmable RNA N(6)-methyladenosine editing by CRISPR-Cas9 conjugates.
Liu XM, Zhou J, Mao Y, Ji Q, Qian SB.
Nat Chem Biol. 2019 Sep;15(9):865-871. doi: 10.1038/s41589-019-0327-1. Epub 2019 Aug 5.
PubMed
Associated Plasmids |
| The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex. Karakkat JV, Kaimala S, Sreedharan SP, Jayaprakash P, Adeghate EA, Ansari SA, Guccione E, Mensah-Brown EPK, Starling Emerald B. Nucleic Acids Res. 2019 Nov 4;47(19):10086-10103. doi: 10.1093/nar/gkz786. PubMed |
| Pathogenic Tau Impairs Axon Initial Segment Plasticity and Excitability Homeostasis. Sohn PD, Huang CT, Yan R, Fan L, Tracy TE, Camargo CM, Montgomery KM, Arhar T, Mok SA, Freilich R, Baik J, He M, Gong S, Roberson ED, Karch CM, Gestwicki JE, Xu K, Kosik KS, Gan L. Neuron. 2019 Nov 6;104(3):458-470.e5. doi: 10.1016/j.neuron.2019.08.008. Epub 2019 Sep 18. PubMed |
| Trimethylamine N-Oxide Binds and Activates PERK to Promote Metabolic Dysfunction. Chen S, Henderson A, Petriello MC, Romano KA, Gearing M, Miao J, Schell M, Sandoval-Espinola WJ, Tao J, Sha B, Graham M, Crooke R, Kleinridders A, Balskus EP, Rey FE, Morris AJ, Biddinger SB. Cell Metab. 2019 Dec 3;30(6):1141-1151.e5. doi: 10.1016/j.cmet.2019.08.021. Epub 2019 Sep 19. PubMed |
| Glucosylceramide synthase maintains influenza virus entry and infection. Drews K, Calgi MP, Harrison WC, Drews CM, Costa-Pinheiro P, Shaw JJP, Jobe KA, Han JD, Fox TE, White JM, Kester M. PLoS One. 2020 Feb 7;15(2):e0228735. doi: 10.1371/journal.pone.0228735. eCollection 2020. PubMed |
| Coxiella effector protein CvpF subverts RAB26-dependent autophagy to promote vacuole biogenesis and virulence. Siadous FA, Cantet F, Van Schaik E, Burette M, Allombert J, Lakhani A, Bonaventure B, Goujon C, Samuel J, Bonazzi M, Martinez E. Autophagy. 2021 Mar;17(3):706-722. doi: 10.1080/15548627.2020.1728098. Epub 2020 Mar 1. PubMed |
| Targeted RNA m(6)A Editing Using Engineered CRISPR-Cas9 Conjugates. Liu XM, Qian SB. Methods Mol Biol. 2021;2298:399-414. doi: 10.1007/978-1-0716-1374-0_23. PubMed |
| Physiological Characterization and Transcriptomic Properties of GnRH Neurons Derived From Human Stem Cells. Keen KL, Petersen AJ, Figueroa AG, Fordyce BI, Shin J, Yadav R, Erdin S, Pearce RA, Talkowski ME, Bhattacharyya A, Terasawa E. Endocrinology. 2021 Sep 1;162(9). pii: 6298609. doi: 10.1210/endocr/bqab120. PubMed |
| Regulation of telomere homeostasis and genomic stability in cancer by N (6)-adenosine methylation (m(6)A). Lee JH, Hong J, Zhang Z, de la Pena Avalos B, Proietti CJ, Deamicis AR, Guzman G P, Lam HM, Garcia J, Roudier MP, Sisk AE, De La Rosa R, Vu K, Yang M, Liao Y, Scheirer J, Pechacek D, Yadav P, Rao MK, Zheng S, Johnson-Pais TL, Leach RJ, Elizalde PV, Dray E, Xu K. Sci Adv. 2021 Jul 28;7(31). pii: 7/31/eabg7073. doi: 10.1126/sciadv.abg7073. Print 2021 Jul. PubMed |
| LncRNA SOX1-OT V1 acts as a decoy of HDAC10 to promote SOX1-dependent hESC neuronal differentiation. Xi J, Xu Y, Guo Z, Li J, Wu Y, Sun Q, Wang Y, Chen M, Zhu S, Bian S, Kang J. EMBO Rep. 2022 Feb 3;23(2):e53015. doi: 10.15252/embr.202153015. Epub 2021 Dec 20. PubMed |
| Podocalyxin-Like Protein 1 Regulates Pluripotency through the Cholesterol Biosynthesis Pathway. Chen WJ, Huang WK, Pather SR, Chang WF, Sung LY, Wu HC, Liao MY, Lee CC, Wu HH, Wu CY, Liao KS, Lin CY, Yang SC, Lin H, Lai PL, Ng CH, Hu CM, Chen IC, Chuang CH, Lai CY, Lin PY, Lee YC, Schuyler SC, Schambach A, Lu FL, Lu J. Adv Sci (Weinh). 2022 Nov 14;10(1):e2205451. doi: 10.1002/advs.202205451. PubMed |
| Protospacer modification improves base editing of a canonical splice site variant and recovery of CFTR function in human airway epithelial cells. Joynt AT, Kavanagh EW, Newby GA, Mitchell S, Eastman AC, Paul KC, Bowling AD, Osorio DL, Merlo CA, Patel SU, Raraigh KS, Liu DR, Sharma N, Cutting GR. Mol Ther Nucleic Acids. 2023 Jun 29;33:335-350. doi: 10.1016/j.omtn.2023.06.020. eCollection 2023 Sep 12. PubMed |
| CRISPR-broad: combined design of multi-targeting gRNAs and broad, multiplex target finding. Veluchamy A, Teles K, Fischle W. Sci Rep. 2023 Nov 12;13(1):19717. doi: 10.1038/s41598-023-46212-x. PubMed |
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