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Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9.
Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC
Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68407PL552Donor empty vector with floxed puromycin resistance gene expression cassette
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68460AAVS1-Neo-CAG-Flpe-ERT2AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and Neo selection gene
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68461AAVS1-Blasticidin-CAG-Flpe-ERT2AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene
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68463Cas9 sgRNA vectorCas9 sgRNA vector for cloning
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68465PAX6 sgRNA2targeting PAX6 gene
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68466PAX6 sgRNA7targeting PAX6 gene
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68467PAX6 CKO 2/7 Donor plasmiddonor plasmid for targeting PAX6 locus
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68468Cas9-2A-Creco-expressing Cas9 and Cre recombinase
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Antibodies from Article