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Cas9 sgRNA vector
(Plasmid #68463)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68463 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    gRNA cloning vector (Addgene plasmid #41824)
  • Backbone size (bp) 3900
  • Modifications to backbone
    A previously described chimeric guide RNA expression cassette was ordered as gBlocks and cloned into the gRNA cloning vector (Addgene plasmid #41824). The new sgRNA cloning vector (Cas9 sgRNA vector) includes two BbsI restriction sites for rapid cloning of sgRNA
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter U6

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

Please follow the sgRNA cloning protocol provided by the Su-Chun Zhang laboratory to use this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cas9 sgRNA vector was a gift from Su-Chun Zhang (Addgene plasmid # 68463 ; http://n2t.net/addgene:68463 ; RRID:Addgene_68463)
  • For your References section:

    Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. 10.1016/j.stem.2015.06.001 PubMed 26145478