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pMJ915
(Plasmid #69090)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69090 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pML-2CT
  • Backbone manufacturer
    MacroLab, UC Berkeley
  • Backbone size w/o insert (bp) 5950
  • Total vector size (bp) 10094
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    5358
  • Promoter T7
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • hexa His (N terminal on backbone)
    • two SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMJ915 was a gift from Jennifer Doudna (Addgene plasmid # 69090)
  • For your References section:

    Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Lin S, Staahl BT, Alla RK, Doudna JA. Elife. 2014 Dec 15;3:e04766. doi: 10.7554/eLife.04766. 10.7554/eLife.04766 PubMed 25497837