pRB1081
(Plasmid
#71479)
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PurposeExpresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRB1017
- Total vector size (bp) 2484
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Vector typeWorm Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedpy-10 gRNA
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gRNA/shRNA sequenceTCCGCTACCATAGGCACCA
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SpeciesSynthetic
- Promoter U6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer M13 forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRB1081 was a gift from Andrew Fire (Addgene plasmid # 71479 ; http://n2t.net/addgene:71479 ; RRID:Addgene_71479) -
For your References section:
Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans. Bell RT, Fu BX, Fire AZ. Genetics. 2015 Dec 17. pii: genetics.115.185041. 10.1534/genetics.115.185041 PubMed 26680661
