pRB1083
(Plasmid
#71480)
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PurposeExpresses VRER Cas9 variant with eef-1A.1 (eft-3) promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCFJ601
- Total vector size (bp) 8113
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Vector typeWorm Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVRER Cas9 variant
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SpeciesSynthetic
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Insert Size (bp)4323
- Promoter eft-3 (eef-1A.1)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRB1083 was a gift from Andrew Fire (Addgene plasmid # 71480 ; http://n2t.net/addgene:71480 ; RRID:Addgene_71480) -
For your References section:
Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans. Bell RT, Fu BX, Fire AZ. Genetics. 2015 Dec 17. pii: genetics.115.185041. 10.1534/genetics.115.185041 PubMed 26680661
