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pMpGE011
(Plasmid #71537)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71537 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMpGWB303
  • Backbone manufacturer
    Kohchi Lab., Kyoto University
  • Backbone size (bp) 13898
  • Modifications to backbone
    Atco_Cas9 CDS and Pea3A terminator were introduced by LR reaction, an then the attR1-attR2 destination cassette was cloned into the Aor51HI site.
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Chlorsulfuron

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Holger Puchta at Karlsruhe Institute of Technology, Germany, for the Atco_Cas9 CDS; Tsuyoshi Nakagawa at Shimane University, Japan for the Gateway cassette.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMpGE011 was a gift from Takayuki Kohchi (Addgene plasmid # 71537 ; http://n2t.net/addgene:71537 ; RRID:Addgene_71537)
  • For your References section:

    Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018. 10.1371/journal.pone.0205117 PubMed 30379827