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Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.
Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
71534pMpGE_En01Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha
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71535pMpGE_En03Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha
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71536pMpGE010For CRISPR/Cas9 genome editing in Marchantia polymorpha
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71537pMpGE011For CRISPR/Cas9 genome editing in Marchantia polymorpha
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108681pMpGE013All-in-one genome editing vector in M. polymorpha (hygromycin resistant)
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108682pMpGE014All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)
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108722pMpGE006Cas9 expression in M.polymorpha
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Recombinant Antibodies from Article