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Gal4-VP16
(Plasmid #71728)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71728 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal4 DBD(1-147)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    441
  • GenBank ID
    NM_001184062.1
  • Tag / Fusion Protein
    • VP16 AD (413-490) (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Steven Triezenberg
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gal4-VP16 was a gift from Lea Sistonen (Addgene plasmid # 71728 ; http://n2t.net/addgene:71728 ; RRID:Addgene_71728)
  • For your References section:

    Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 from Its Activation. Budzynski MA, Puustinen MC, Joutsen J, Sistonen L. Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. Epub 2015 May 11. 10.1128/MCB.00816-14 PubMed 25963659