pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
(Plasmid #71761)

• Purpose: mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons
• Depositing Lab: Liqun Luo
• Publication: Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10.1016/j.cell.2015.07.015.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4941
• Total vector size (bp): 7404
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mGFP
• Insert Size (bp): 777
• Tag / Fusion Protein:
  • Palmitoylation signal (N terminal on insert)

Gene/Insert 2

• Gene/Insert name: Synaptophysin
• Insert Size (bp): 951
• Tag / Fusion Protein:
  • mRuby (C terminal on insert)

Resource Information

• Supplemental Documents:
  • Sequence file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry