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pFETCh_ZNF219
(Plasmid #72367)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72367 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFETCh_Donor
  • Backbone size w/o insert (bp) 5771
  • Vector type
    CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZNF219 Homology Arms
  • Species
    H. sapiens (human)
  • Entrez Gene
    ZNF219 (a.k.a. ZFP219)
  • Tag / Fusion Protein
    • 3XFLAG-P2A-NeoR (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Addgene's quality control sequencing finds a G to E amino acid residue substitution in the linker between ZNF219 and the 3xFlag. This residue change is not thought to affect plasmid function. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFETCh_ZNF219 was a gift from Eric Mendenhall & Richard M. Myers (Addgene plasmid # 72367 ; http://n2t.net/addgene:72367 ; RRID:Addgene_72367)