|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72367||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5771
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameZNF219 Homology Arms
SpeciesH. sapiens (human)
Entrez GeneZNF219 (a.k.a. ZFP219)
/ Fusion Protein
- 3XFLAG-P2A-NeoR (C terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer Neo-F
- 3′ sequencing primer Neo-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Addgene's quality control sequencing finds a G to E amino acid residue substitution in the linker between ZNF219 and the 3xFlag. This residue change is not thought to affect plasmid function. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFETCh_ZNF219 was a gift from Eric Mendenhall & Richard M. Myers (Addgene plasmid # 72367 ; http://n2t.net/addgene:72367 ; RRID:Addgene_72367)