|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72369||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9289
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
- Cloning method Gibson Cloning
- 5′ sequencing primer pBR322ori-F (Common Sequencing Primers)
Terms and Licenses
Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PX458_ZNF219_2 was a gift from Eric Mendenhall & Richard M. Myers (Addgene plasmid # 72369 ; http://n2t.net/addgene:72369 ; RRID:Addgene_72369)