|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72640||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDONR 221
- Total vector size (bp) 11596
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameelavl3 enhancer
SpeciesD. rerio (zebrafish)
Insert Size (bp)8742
Entrez Geneelavl3 (a.k.a. HuC, elrc, id:ibd1248, wu:fb77b03, zHuC)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe p5E-elavl3 enhancer clone was generated by inserting the XhoI/SalI fragment from pCS2-HuC:Kaede (Sato et al., 2006) (Hitoshi Okamoto lab) into the XhoI site of p5E-MCS.
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL. Gal80 intersectional regulation of cell-type specific expression in vertebrates. Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8. PubMed PMID:21905164; PubMed Central PMCID: PMC3178006.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p5E-elavl3 was a gift from Joshua Bonkowsky (Addgene plasmid # 72640 ; http://n2t.net/addgene:72640 ; RRID:Addgene_72640)