pEGFP-hGal3
(Plasmid #73080)

• Purpose: Expression in mammalian cells
• Depositing Lab: Tamotsu Yoshimori
• Publication: Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi: 10.1038/emboj.2013.171. Epub 2013 Aug 6.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5460
• Modifications to backbone: No major modification to backbone. Digested with Bgl1+Sal1 to add the insert cDNA.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Galectin-3
• Alt name: LGALS3
• Species: H. sapiens (human)
• Insert Size (bp): 754
• Entrez Gene: LGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl2 (not destroyed)
• 3′ cloning site: Sal1 (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is used to detect ruptured lysosomal membrane by lysosomotropic agents or endosomal membrane accompanied with invading bacteria.