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PurposepZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74062 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepZ8-1
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Modifications to backboneAdded LacIq repressor
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedcas9
- Promoter ptac
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGACATCATAACGGTTCTGGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid used to repress genes in Corynebacterium glutamicum as described in Cleto et al., 2016. Note that the species of dCas9 in this plasmid is S. pyogenes.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZ8-T_dCas9 was a gift from Timothy Lu (Addgene plasmid # 74062 ; http://n2t.net/addgene:74062 ; RRID:Addgene_74062) -
For your References section:
Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). Cleto S, Jensen JV, Wendisch VF, Lu TK. ACS Synth Biol. 2016 Feb 16. 10.1021/acssynbio.5b00216 PubMed 26829286
Map uploaded by the depositor.
