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Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
Cleto S, Jensen JV, Wendisch VF, Lu TK
ACS Synth Biol. 2016 Feb 16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
74062pZ8-T_dCas9pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR
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74063pZ8-P_dCas9pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
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74064pZ8-PtacIPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR
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74065pZ8-PrppZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanR
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74066pAL-pgi_TpAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR
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74067pAL-pgi_NTpAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR
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74068pAL-pck_TpAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR
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74069pAL-pck_NTpAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR
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74070pAL-pyk_TpAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecR
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74072pAL-pyk_NTpAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR
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74073pRIM3-rfpGentR version of pRIM2 carrying rfp
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74075pAL-rfp_TpAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR
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79642Corynebacterium glutamicum::RFPExpresses RFP from the chromosome of Corynebacterium glutamicum ATCC 13032 (ptac promoter unrepressed; no LacI present)
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