|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74072||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Gene/Insert namesgRNA pyk (NT)
gRNA/shRNA sequencepyk (NT)
- Promoter ptac
- Cloning method Gibson Cloning
- 5′ sequencing primer GGCAGCCATCGGAAGCTGTGG (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAL-pyk_NT was a gift from Timothy Lu (Addgene plasmid # 74072 ; http://n2t.net/addgene:74072 ; RRID:Addgene_74072)
For your References section:Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). Cleto S, Jensen JV, Wendisch VF, Lu TK. ACS Synth Biol. 2016 Feb 16. 10.1021/acssynbio.5b00216 PubMed 26829286