PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74448||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2710
- Total vector size (bp) 2850
Growth in Bacteria
Copy numberHigh Copy
Alt nameH2O-forming NADH oxidase from Lactobacillus brevis
Insert Size (bp)1490
MutationHuman codon optimized
/ Fusion Proteins
- Flag tag with linker (C terminal on insert)
- mitochondrial targeting sequence (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (not destroyed)
- 3′ cloning site Mlu1 (not destroyed)
- 5′ sequencing primer GTAAAACGACGGCCAGTG
- 3′ sequencing primer GGAAACAGCTATGACCATG (Common Sequencing Primers)
Terms and Licenses
In our original publication we express mitoLbNOX in mammalian cells using a Tet3G dox-inducible system from Clontech. We cannot distribute those exact plasmids due to Clontech license restrictions. We recommend that investigators subclone this mitoLbNOX sequence into their preferred expression system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUC57-mitoLbNOX was a gift from Vamsi Mootha (Addgene plasmid # 74448 ; http://n2t.net/addgene:74448 ; RRID:Addgene_74448)
For your References section:Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio. Titov DV, Cracan V, Goodman RP, Peng J, Grabarek Z, Mootha VK. Science. 2016 Apr 8;352(6282):231-5. doi: 10.1126/science.aad4017. Epub 2016 Apr 7. 10.1126/science.aad4017 PubMed 27124460