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pUC57-TPNOX
(Plasmid #87853)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87853 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    GeneWiz
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 4131
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TPNOX
  • Alt name
    NADPH specific mutant of LbNOX
  • Species
    H.sapiens codon optimized bacterial gene
  • Insert Size (bp)
    1421
  • Tag / Fusion Protein
    • C-terminal FLAG tag with a linker (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer TGT AAA ACG ACG GCC AGT
  • 3′ sequencing primer CAG GAA ACA GCT ATG AC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-TPNOX was a gift from Vamsi Mootha (Addgene plasmid # 87853 ; http://n2t.net/addgene:87853 ; RRID:Addgene_87853)
  • For your References section:

    A genetically encoded tool for manipulation of NADP+/NADPH in living cells. Cracan V, Titov DV, Shen H, Grabarek Z, Mootha VK. Nat Chem Biol. 2017 Aug 7. doi: 10.1038/nchembio.2454. 10.1038/nchembio.2454 PubMed 28805804