pUC57-mitoTPNOX
(Plasmid #87854)

• Purpose: This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
• Depositing Lab: Vamsi Mootha
• Publication: Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/nchembio.2454.

Backbone

• Vector backbone: pUC57
• Backbone manufacturer: GeneWiz
• Backbone size w/o insert (bp): 2710
• Total vector size (bp): 4200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitoTPNOX
• Alt name: NADPH specific mutant of mitoLbNOX
• Species: H.sapiens codon optimized bacterial gene
• Insert Size (bp): 1490
• Tags / Fusion Proteins:
  • C-terminal FLAG tag with a linker (C terminal on insert)
  • mitochondrial targeting sequence (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: TGT AAA ACG ACG GCC AGT
• 3′ sequencing primer: CAG GAA ACA GCT ATG AC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry