Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #87854)


Item Catalog # Description Quantity Price (USD)
Plasmid 87854 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 4200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    NADPH specific mutant of mitoLbNOX
  • Species
    H.sapiens codon optimized bacterial gene
  • Insert Size (bp)
  • Tags / Fusion Proteins
    • C-terminal FLAG tag with a linker (C terminal on insert)
    • mitochondrial targeting sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer TGT AAA ACG ACG GCC AGT
  • 3′ sequencing primer CAG GAA ACA GCT ATG AC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57-mitoTPNOX was a gift from Vamsi Mootha (Addgene plasmid # 87854 ; ; RRID:Addgene_87854)
  • For your References section:

    A genetically encoded tool for manipulation of NADP+/NADPH in living cells. Cracan V, Titov DV, Shen H, Grabarek Z, Mootha VK. Nat Chem Biol. 2017 Aug 7. doi: 10.1038/nchembio.2454. 10.1038/nchembio.2454 PubMed 28805804