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pBluescriptSKII+ U6-sgRNA(F+E) ACTB
(Plasmid #74705)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74705 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
  • gRNA/shRNA sequence
    3'UTR of ACTB mRNA

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescriptSKII+ U6-sgRNA(F+E) ACTB was a gift from Eugene Yeo (Addgene plasmid # 74705 ; http://n2t.net/addgene:74705 ; RRID:Addgene_74705)
  • For your References section:

    Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, Yeo GW. Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 10.1016/j.cell.2016.02.054. 10.1016/j.cell.2016.02.054 PubMed 26997482