Holiday Schedule: Addgene will be closed December 22nd & 25th and January 1st. For details, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

CIB1-CreC(N1)
(Plasmid #75367)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 75367 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mCherryC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    Contains IRES between mCherry and CIB1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIB1
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Cre DNA recombinase, residues 106-343 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CIB1-CreC(N1) was a gift from Chandra Tucker (Addgene plasmid # 75367)
  • For your References section:

    Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase. Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL. Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063. 10.1038/nchembio.2063 PubMed 27065233