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Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase.
Taslimi A, Zoltowski B, Miranda JG, Pathak GP, Hughes RM, Tucker CL
Nat Chem Biol. 2016 Apr 11. doi: 10.1038/nchembio.2063.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
75366CIB81-pmGFPExpresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end
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75367CIB1-CreC(N1)Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase
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75368CRY2(L348F)-CreNExpresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase
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75369Gal4BD-CRY2(535)Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)
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75370CRY2PHR(W349R)-mCherryExpresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry
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75372CRY2(535, L348F)-mCherryExpresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry
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