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Kras-Src FRET biosensor
(Plasmid #78302)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78302 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Src biosensor
  • Alt name
    ECFP-SH2-linker-substrate-YPet-KRasTag
  • Species
    Synthetic
  • Insert Size (bp)
    1800
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Kras-Src FRET biosensor was a gift from Yingxiao Wang (Addgene plasmid # 78302 ; http://n2t.net/addgene:78302 ; RRID:Addgene_78302)
  • For your References section:

    Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors. Ouyang M, Sun J, Chien S, Wang Y. Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14353-8. doi: 10.1073/pnas.0807537105. Epub 2008 Sep 17. 10.1073/pnas.0807537105 PubMed 18799748