Cas9-GFP_sg_mTfeb
(Plasmid #79006)

• Purpose: Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.
• Depositing Lab: Reuben Shaw
• Publication: Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi: 10.1101/gad.274142.115.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	79006	Standard format: Plasmid sent in bacteria as agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pSpCas9(BB)-2A-GFP (PX458)
  (Search Vector Database)
• Total vector size (bp): 9300
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sgTfeb
• gRNA/shRNA sequence: AGCACTGTTGCCGGCCGAGG
• Species: M. musculus (mouse)
• Entrez Gene: Tfeb (a.k.a. Tcfeb, bHLHe35)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: GAGGGCCTATTTCCCATGATTCC

Resource Information

• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials