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pscAAV-CAG-RLuc
(Plasmid #83280)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83280 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV with one trs deleted ITR and CAG promoter
  • Backbone size w/o insert (bp) 4105
  • Total vector size (bp) 5041
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Need to check for integrity of ITRs by restriction digest with AhdI, also with XmaI.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla Luciferase
  • Species
    Renilla reniformis
  • Insert Size (bp)
    936
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EagI (not destroyed)
  • 5′ sequencing primer GTTATTGTGCTGTCTCATC
  • 3′ sequencing primer CCACACCTCCCCCTGAAC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    AAV backbone was obtained from Addgene plasmid #32396, the CAG promoter was obtained from Addgene plasmid #37825, renilla luciferase gene was obtained from Promega plasmid pRL-TK
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pscAAV-CAG-RLuc was a gift from Mark Kay (Addgene plasmid # 83280 ; http://n2t.net/addgene:83280 ; RRID:Addgene_83280)
  • For your References section:

    Bioengineered Viral Platform for Intramuscular Passive Vaccine Delivery to Human Skeletal Muscle. Paulk NK, Pekrun K, Charville GW, Maguire-Nguyen K, Wosczyna MN, Xu J, Zhang Y, Lisowski L, Yoo B, Vilches-Moure JG, Lee GK, Shrager JB, Rando TA, Kay MA. Mol Ther Methods Clin Dev. 2018 Jul 24;10:144-155. doi: 10.1016/j.omtm.2018.06.001. eCollection 2018 Sep 21. 10.1016/j.omtm.2018.06.001 PubMed 30101152