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pAAV-CAG-FLuc
(Plasmid #83281)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83281 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV with CAG promoter
  • Backbone size w/o insert (bp) 4721
  • Total vector size (bp) 6374
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Need to check for integrity of ITRs by restriction digest with AhdI, also with XmaI.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly Luciferase
  • Species
    Photinus pyralis (Firefly)
  • Insert Size (bp)
    1653
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer GTTATTGTGCTGTCTCATC
  • 3′ sequencing primer CATTAAAGCAGCGTATCC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    AAV backbone as well as CAG promoter and WPRE sequences were obtained from Addgene plasmid #37825, sequences for the FLuc gene were obtained from Addgene plasmid #21375
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-FLuc was a gift from Mark Kay (Addgene plasmid # 83281 ; http://n2t.net/addgene:83281 ; RRID:Addgene_83281)
  • For your References section:

    Bioengineered Viral Platform for Intramuscular Passive Vaccine Delivery to Human Skeletal Muscle. Paulk NK, Pekrun K, Charville GW, Maguire-Nguyen K, Wosczyna MN, Xu J, Zhang Y, Lisowski L, Yoo B, Vilches-Moure JG, Lee GK, Shrager JB, Rando TA, Kay MA. Mol Ther Methods Clin Dev. 2018 Jul 24;10:144-155. doi: 10.1016/j.omtm.2018.06.001. eCollection 2018 Sep 21. 10.1016/j.omtm.2018.06.001 PubMed 30101152