Addgene - GST-FOXO3a TM 1-506aa Plasmid Data

Plasmid 8351: GST-FOXO3a TM 1-506aa

Gene/insert name:
FOXO3a 1-506aa
Alt name:
FKHRL1
Alt name:
forkhead family
Insert size:
1500
Species:
H. sapiens (human)
Entrez Gene:
FOXO3 (AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Mutation:
T32A, S253A, S315A
Vector backbone:
pGex 4T3
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
4968
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Michael Greenberg
Terms and Licenses:
MTA

Comments:

Note that the stop codon in the vector backbone is used to terminate translation. Thus, a 10aa sequence (VDSSGRIVTD) will be expressed as part of FOXO3a.

See GST-FOXO3a 1-506aa for wild-type map and sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Brunet et al (Cell 1999 Mar 19;96(6):857-68. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8351" in your Materials and Methods section.